ディテクター（検出器）としては目的とする物質の性質に応じて光学的性質（吸光度、屈折率、蛍光等）、電気化学的性質、質量分析法などを利用する装置がある。
각각 다른 산업 분야에 대한 자세한 정보 및 다양한 카테고리는 다음 써모 피셔 사이언티픽 학습 센터에서 산업 및 응용 과학 페이지를 확인하세요.
예를 들어 설탕과 같이 물에 녹기 쉬운 물질을 첨가했을 때 설탕은 기름층에 거의 녹지 않으므로 물층에 많이 존재하게 됩니다. 반대로 식용유와 같이 헥산에 녹기 쉬운 용질을 첨가했을 때는 물층보다 기름층에 많이 존재합니다. 이와같이, 설탕과 식용유는 물과 헥산의 두 상 사이의 존재의 비율(=분배 비율)이 크게 다르기 때문에, 만약 당신과 이 분액깔대기에서 설탕만을 분리하고 싶다면, 분액깔대기에서 물층만을 꺼내 물을 증류시키면 설탕만을 얻을 수 있습니다.
物質の電気化学的な性質を利用した検出器。pHの変動や酸化還元電位の変動を用いて測定を行う。
a values, the pH with the mobile phase has another effect on Every solute’s retention time, allowing for us to find the the best possible pH for effecting a whole separation on the 4 solutes.
An inner typical is essential when making use of HPLC–MS because the interface amongst the HPLC plus the mass spectrometer won't let for your reproducible transfer with the column’s eluent in to the MS’s ionization chamber.
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The elution get of solutes in HPLC is governed by polarity. For a standard-period separation, a solute of lessen polarity spends proportionally much less time inside the polar stationary period and elutes just before a solute that's additional polar. Presented a certain stationary HPLC working stage, retention periods in ordinary-phase HPLC are controlled by adjusting the mobile period’s Homes. For example, if the resolution between two solutes is very poor, switching to some considerably less polar mobile phase keeps the solutes on the column for an extended time and offers additional prospect for their separation.
Different types of detectors used in HPLC are refractive index detectors, UV detectors, and fluorimetry detectors.
The most well-liked HPLC detectors benefit from an analyte’s UV/Vis absorption spectrum. These detectors range between simple layouts, where the analytical wavelength is chosen using proper filters, into a modified spectrophotometer where the sample more info compartment includes a flow mobile.
The column is definitely the separation chamber where the magic of HPLC happens. It houses the stationary section, a packed bed of microscopic particles.
溶媒の組成に勾配を付けて（すなわち組成を連続的に変えて）溶出を行うことも多い。たとえば後述の逆相クロマトグラフィーにおいて水/メタノール勾配を使う場合、まずメタノールの少ない条件で極性の高い物質が溶出し、その後メタノールの割合を増加させてゆくに従ってより極性の低い物質が順次溶出する。これをグラジェント分析と呼ぶ。これに対し、一定組成の溶媒で分析物を溶出させる分析法をアイソクラテック分析と呼ぶ。
The selection of detector depends on the precise requires from the Evaluation, thinking about aspects like sensitivity, selectivity, and compatibility With all the mobile section.
The injector introduces a exact quantity with the sample Resolution in to the cell period stream. A number of injection methods exist, with loop injection remaining a typical technique.